Services
Affinity Selection Mass Spectrometry (ASMS)
ASMS offers several distinct advantages over legacy high throughput screening (HTS) methods:
- ASMS is binding site agnostic, enabling clients to identify potential leads acting through a variety of mechanisms, including degradation and disruption of protein-protein or protein-oligonucleotide interactions.
- ASMS is compatible with a range of targets. Protein, RNA, and Protein Complexes can be screened without the need for tagging or surface immobilization, ensuring the results are physiologically relevant.
- ASMS requires very little target (~1mg) to screen hundreds of thousands of potential compounds.
- ASMS assay development is limited to buffer optimization and therefore much faster than traditional biochemical HTS methods like FRET and enzymatic assays, enabling identification of leads in half the time.
- ASMS is much faster to run than other biophysical assays such as SPR, interferometry, DSF, Mesoscale, ITC, and NMR.
- ASMS allows compounds to be screened in pools of 250: 100,000 test compounds can be screened in a single day.
- ASMS covers broad chemical space as positive mode electrospray ionization is compatible with 95% of drug-like test compounds.
- ASMS is immune to false positives resulting from active impurities and breakdown products by identifying the exact compound based on molecular formula.
We can our in-house library, third-party libraries, and internal client libraries. While we typically screen Rule of Five compliant chemical libraries, we can also screen macrocyclics. We offer target QC on proteins and RNA to ensure accurate screens.
Following ASMS primary, pooled screens:
- We recommend clients run a secondary screen to confirm hits in small pools of one to five compounds.
- We can also rank order hits by competitive binding, give quantitative information on target occupancy, and determine binding affinity for Structure-Activity Relationship (SAR) studies.
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Covalent Binding Assays (Protein & RNA)
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Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
- We understand that ICP-MS results are crucial to go-forward decision making in CGT and biologics development, so we strive to turn around data as fast as possible. We can analyze hundreds of samples daily.
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Small Molecule Analysis
Small Molecule Analysis Assays
- Biomarker validation and screening from cell culture, biological fluids, or tissue
- Functional assay design and development for rare diseases
- Potency assays
- Small molecule library QC
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RapidFire-MS High Throughput Functional Assays
- As the inventors of the RapidFire-MS platform, we have 20 years of experience in RapidFire analytical method development and biochemical assay development.
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Oligo Analysis
Services at a glance
- Mass determination
- Purity analysis
- Impurities analysis
- Covalent modification
- RNA cap and tail analyses
- Sequencing
Instrumentation
- TOF and QTOF mass spectrometers
- LC and RapidFire High-throughput Systems
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Proteomics/Chemoproteomics
Following covalent ligand discovery, Momentum’s proteomic assays can determine the site and occupancy of compound modification on the protein. Activity based protein profiling can be used to identify on- and off-target interactions in vivo on a proteome-wide scale.
Following the identification of reversible small molecule binders by the ASMS assay, Momentum’s proteomic assays can, for examples, measure their effect on splicing, post translational modifications, or targeted protein degradation.
Together, Momentum’s deep expertise in screening assays and quantitative multiplexed proteomics allows our clients to progress from target of interest to compounds with cellular activity in just a few months.
Services at a glance
- Protein mass and purity analysis
- Protein identification
- Sequence confirmation
- Covalent modification mapping
- Covalent protein binding in vivo (ABPP)
- Global quantitative profiling in vivo
- Kinase profiling in vivo
- PTM analysis in vivo
Instrumentation
- Orbitrap Ascend, QTOF, and QQQ mass spectrometers
- Extensive automation for sample preparation
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Explore proteomic datasets using our dynamic web application
Check out some of our covalent ligandability data jointly presented with Moma Therapeutics at ASMS 2024
Read our blog post on targeted protein degraders/PROTACs here
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