CHIPP

While CHIPP is performed on cell extracts, we offer an alternative target identification approach, SPICE, that is designed for the detection of drug:target interactions in live cells.

CHIPP is a flexible, scalable platform that can accommodate everything from a single target analysis to the interrogation of hundreds of primary screening hits. Our team is happy to discuss specific details regarding turnaround time for larger-scale experiments.

CHIPP has demonstrated a high success rate for the analysis of many compound types, including allosteric inhibitors, molecular glues, PROTACs and other TPD modalities, and enzyme inhibitors. If you have questions about the compatibility of your specific compound(s) of interest, please contact our team.

While both approaches detect changes in protein stability upon compound binding as a signal of target engagement, CHIPP samples are denatured by exposure to an increasing gradient of chaotrope salts, rather than by increasing temperatures. Because CHIPP is a bead-based protocol, experiment throughput is significantly increased, allowing the automated analysis of hundreds of samples at a reasonable cost and turnaround time.

A typical experiment readily enables profiling of >8,000 proteins thanks to its proteomic readout.

CHIPP can be used for any application involving target identification, deconvolution, or selectively profiling. Thanks to its high throughput, this approach is well-suited as an early-stage follow-up assay for primary screening hits, especially those identified through phenotypic screens. The technology can be used to monitor ternary complex formation for the characterization of molecular glues and targeted protein degraders. CHIPP also provides valuable information about proteome-wide off-target interactions, de-risking compounds early in the drug discovery and development process.