KinomeScout™

KinomeScout™ is a versatile and well-established tool for kinome profiling that works with native proteins under physiological conditions to direct the design of potent and selective small-molecule drug candidates, while circumventing typical downsides of protein kinase panel assays. The technology is based on proprietary kinase-binding matrices that can be used to profile over 300 different kinases and other ATP binding proteins (such as helicases or phospho-diesterases). Thanks to our Kinobeads-like chemical proteomics approach, kinase inhibitors can be profiled without the need for labels on compounds or proteins. The KinomeScout™ technology enables selectivity profiling by testing hits, leads and drug candidates against all kinases in a tissue or cell type in a single experiment, facilitating identification of off-targets and providing physiologically relevant selectivity and affinity information (such as EC₅₀ and estimated K_d values).

We offer multiple workflow options to match your experimental needs. The assay is also readily available as KinomeScout™ HT format for profiling 1,000 to 10,000s of protein kinase inhibitors in a reasonable timeframe.

We can also profile kinase inhibitors and other ATP-binding proteins using activity-based profiling approaches, such as ActivX (KiNative™).